Composite

Part:BBa_I712039

Designed by: Katja Kolar   Group: iGEM07_Ljubljana   (2007-10-24)


CMVp - HA - CD4 - CUb - GFP

CD4 with HA-tag (after the signal peptide) fused with C-terminal half of split-ubiquitin system and mCerulean.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1307
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2095
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1920
    Illegal NgoMIV site found at 1969
    Illegal AgeI site found at 2050
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1754
    Illegal SapI.rc site found at 842


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Categories
Parameters
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